[Radioimmunological analysis of the neuraminidase of influenza virus subtype N2].
Identifieur interne : 002D93 ( Main/Exploration ); précédent : 002D92; suivant : 002D94[Radioimmunological analysis of the neuraminidase of influenza virus subtype N2].
Auteurs : A K Gitel'Man ; Iu A. Zakomyrdin ; I G KharitonenkovSource :
- Voprosy virusologii [ 0507-4088 ]
Descripteurs français
- KwdFr :
- Animaux, Antigènes de surface (analyse), Antigènes viraux (analyse), Dosage radioimmunologique (), Immunisation, Lapins, Sialidase (analyse), Sialidase (immunologie), Sialidase (isolement et purification), Virus de la grippe A (enzymologie), Virus de la grippe A (immunologie), Électrophorèse sur gel de polyacrylamide.
- MESH :
- analyse : Antigènes de surface, Antigènes viraux, Sialidase.
- enzymologie : Virus de la grippe A.
- immunologie : Sialidase, Virus de la grippe A.
- isolement et purification : Sialidase.
- Animaux, Dosage radioimmunologique, Immunisation, Lapins, Électrophorèse sur gel de polyacrylamide.
English descriptors
- KwdEn :
- Animals, Antigens, Surface (analysis), Antigens, Viral (analysis), Electrophoresis, Polyacrylamide Gel, Immunization, Influenza A virus (enzymology), Influenza A virus (immunology), Neuraminidase (analysis), Neuraminidase (immunology), Neuraminidase (isolation & purification), Rabbits, Radioimmunoassay (methods).
- MESH :
- chemical , analysis : Antigens, Surface, Antigens, Viral, Neuraminidase.
- enzymology : Influenza A virus.
- immunology : Influenza A virus, Neuraminidase.
- chemical , isolation & purification : Neuraminidase.
- methods : Radioimmunoassay.
- Animals, Electrophoresis, Polyacrylamide Gel, Immunization, Rabbits.
Abstract
Competitive radioimmunoassay in the homologous system using 125I-labeled neuraminidase (NA) of A/Japan/305/57 virus and antibodies to it showed that influenza viruses NA of subtypes H2N2 (A/Singapore/1/57 and A/Tokyo/3/67) and H3N2 (A/Aichi/2/68, A/England/42/72, A/Port Chalmers/1/73, and A/Texas/1/77) had undergone clear antigenic change despite the presence of Japan strain NA determinant. The use of a heterologous system (125I-NA of the Japan strain--antibodies to NA of England/42/72 strain) for the study of the intratype determinant showed the presence of a common determinant in all the strains under study, this determinant being represented dissimilarly, however. Neuraminidase of the Texas strain has a determinant differing from the intratype one, common for the Japan and England viruses.
PubMed: 6659471
Affiliations:
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Le document en format XML
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<series><title level="j">Voprosy virusologii</title>
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<term>Antigens, Viral (analysis)</term>
<term>Electrophoresis, Polyacrylamide Gel</term>
<term>Immunization</term>
<term>Influenza A virus (enzymology)</term>
<term>Influenza A virus (immunology)</term>
<term>Neuraminidase (analysis)</term>
<term>Neuraminidase (immunology)</term>
<term>Neuraminidase (isolation & purification)</term>
<term>Rabbits</term>
<term>Radioimmunoassay (methods)</term>
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<keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term>
<term>Antigènes de surface (analyse)</term>
<term>Antigènes viraux (analyse)</term>
<term>Dosage radioimmunologique ()</term>
<term>Immunisation</term>
<term>Lapins</term>
<term>Sialidase (analyse)</term>
<term>Sialidase (immunologie)</term>
<term>Sialidase (isolement et purification)</term>
<term>Virus de la grippe A (enzymologie)</term>
<term>Virus de la grippe A (immunologie)</term>
<term>Électrophorèse sur gel de polyacrylamide</term>
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<keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>Antigens, Surface</term>
<term>Antigens, Viral</term>
<term>Neuraminidase</term>
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<keywords scheme="MESH" qualifier="analyse" xml:lang="fr"><term>Antigènes de surface</term>
<term>Antigènes viraux</term>
<term>Sialidase</term>
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<keywords scheme="MESH" qualifier="enzymologie" xml:lang="fr"><term>Virus de la grippe A</term>
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<keywords scheme="MESH" qualifier="enzymology" xml:lang="en"><term>Influenza A virus</term>
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<keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Sialidase</term>
<term>Virus de la grippe A</term>
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<keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Influenza A virus</term>
<term>Neuraminidase</term>
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<keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Sialidase</term>
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<term>Electrophoresis, Polyacrylamide Gel</term>
<term>Immunization</term>
<term>Rabbits</term>
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<term>Dosage radioimmunologique</term>
<term>Immunisation</term>
<term>Lapins</term>
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<front><div type="abstract" xml:lang="en">Competitive radioimmunoassay in the homologous system using 125I-labeled neuraminidase (NA) of A/Japan/305/57 virus and antibodies to it showed that influenza viruses NA of subtypes H2N2 (A/Singapore/1/57 and A/Tokyo/3/67) and H3N2 (A/Aichi/2/68, A/England/42/72, A/Port Chalmers/1/73, and A/Texas/1/77) had undergone clear antigenic change despite the presence of Japan strain NA determinant. The use of a heterologous system (125I-NA of the Japan strain--antibodies to NA of England/42/72 strain) for the study of the intratype determinant showed the presence of a common determinant in all the strains under study, this determinant being represented dissimilarly, however. Neuraminidase of the Texas strain has a determinant differing from the intratype one, common for the Japan and England viruses.</div>
</front>
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<affiliations><list></list>
<tree><noCountry><name sortKey="Gitel Man, A K" sort="Gitel Man, A K" uniqKey="Gitel Man A" first="A K" last="Gitel'Man">A K Gitel'Man</name>
<name sortKey="Kharitonenkov, I G" sort="Kharitonenkov, I G" uniqKey="Kharitonenkov I" first="I G" last="Kharitonenkov">I G Kharitonenkov</name>
<name sortKey="Zakomyrdin, Iu A" sort="Zakomyrdin, Iu A" uniqKey="Zakomyrdin I" first="Iu A" last="Zakomyrdin">Iu A. Zakomyrdin</name>
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