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[Radioimmunological analysis of the neuraminidase of influenza virus subtype N2].

Identifieur interne : 002D93 ( Main/Exploration ); précédent : 002D92; suivant : 002D94

[Radioimmunological analysis of the neuraminidase of influenza virus subtype N2].

Auteurs : A K Gitel'Man ; Iu A. Zakomyrdin ; I G Kharitonenkov

Source :

RBID : pubmed:6659471

Descripteurs français

English descriptors

Abstract

Competitive radioimmunoassay in the homologous system using 125I-labeled neuraminidase (NA) of A/Japan/305/57 virus and antibodies to it showed that influenza viruses NA of subtypes H2N2 (A/Singapore/1/57 and A/Tokyo/3/67) and H3N2 (A/Aichi/2/68, A/England/42/72, A/Port Chalmers/1/73, and A/Texas/1/77) had undergone clear antigenic change despite the presence of Japan strain NA determinant. The use of a heterologous system (125I-NA of the Japan strain--antibodies to NA of England/42/72 strain) for the study of the intratype determinant showed the presence of a common determinant in all the strains under study, this determinant being represented dissimilarly, however. Neuraminidase of the Texas strain has a determinant differing from the intratype one, common for the Japan and England viruses.

PubMed: 6659471


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Le document en format XML

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<term>Electrophoresis, Polyacrylamide Gel</term>
<term>Immunization</term>
<term>Influenza A virus (enzymology)</term>
<term>Influenza A virus (immunology)</term>
<term>Neuraminidase (analysis)</term>
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<term>Antigens, Surface</term>
<term>Antigens, Viral</term>
<term>Neuraminidase</term>
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<term>Antigènes de surface</term>
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<term>Virus de la grippe A</term>
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<term>Influenza A virus</term>
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<term>Influenza A virus</term>
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<div type="abstract" xml:lang="en">Competitive radioimmunoassay in the homologous system using 125I-labeled neuraminidase (NA) of A/Japan/305/57 virus and antibodies to it showed that influenza viruses NA of subtypes H2N2 (A/Singapore/1/57 and A/Tokyo/3/67) and H3N2 (A/Aichi/2/68, A/England/42/72, A/Port Chalmers/1/73, and A/Texas/1/77) had undergone clear antigenic change despite the presence of Japan strain NA determinant. The use of a heterologous system (125I-NA of the Japan strain--antibodies to NA of England/42/72 strain) for the study of the intratype determinant showed the presence of a common determinant in all the strains under study, this determinant being represented dissimilarly, however. Neuraminidase of the Texas strain has a determinant differing from the intratype one, common for the Japan and England viruses.</div>
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<name sortKey="Zakomyrdin, Iu A" sort="Zakomyrdin, Iu A" uniqKey="Zakomyrdin I" first="Iu A" last="Zakomyrdin">Iu A. Zakomyrdin</name>
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